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Theoretical consideration was made to make an economical and rational method of samplinginspection for pebrine control by making use of the characteristic of early eclosion of silkworm moths diseased with pebrine of a lot, and the description was made on the five factors of investigations which are considered to be indispensable and sufficient for establishing a new sampling inspection method:-1. Infection formula of pebrine disease.2. Relationship between eclosion dates and percentage of diseased moths of a lot.In this item, there include experiments which are performed in all cases which have from the case of heavily infected moths with high, diseased percentage to the case of the very lightly infected, and in an unhealthy lot.3. Comparison of descending grade of percentages of diseased moths among heavily to lightly infected moths accrding to the retardation of eclosion dates by the results of experiments 2.4. Comparison between weights of healthy undried cocoon, its silk layer and those of the diseased respectively.5. Investigation on the hereditary transportation of parasites into silkworm eggs.5A. The case of hibernating eggs.5B. Comparison between common acid treated eggs and those of the chilled and the acid treated.In order to perform above experiments, it is necessary to make moths with various desired percentages of diseased ones infected with pebrine at ones discretion. Therefore, the results of experiment on the numerical infection of spores to a silkworm larva per os which gave satisfactory answer were described. By this and the other correlated experiments, it became easy to obtain moths, apparently healthy despite infected with pebrine, and those with any desired disease percentages by oral infection at any instar.

nihon ouyou doubutsu konchuu gakkaishi/nihon ōyō dōbutsu konchū gakkaishi

Experimental Design on a Sampling Inspection Method of Pébrine Control by Utilizing the Nature Characterized by Early Eclosion of Diseased Silkworms with Pébrine of a Lot