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succeeding growth ofSteinemema.fettiae (DD-136) in SPodoPtera literra l rvae. At an early time (3 hr) after inoculation, the infective juveniles were not always observed on the mouth or anus of a still active insect. Nematode invasion occurred as promptly as in 1 hr after inoculation with ca. 1,OOOjuveniles and then increased with time. Twelve to 24 hr after inoculation, more nematodes were obseTved in hemocoel than in alimentary canal, cspecially in the hernocoel lying beneath the deeply invaginated intersegmental membrancs, Generally, the most developed nematodes were in the insect head, Nematodes grew rapidly from 24 to 36 hr after inoculation, accompanied by the drastic disintegration ofinsect tissues in which they often ernbedded themselves. SEM observations clearly revealed a nematode Iiving on and in a larva and also a nematode feeding on rather Iarge pieces of various insect tissues.

applied entomology and zoology

Histological and SEM Observations on the Invasion and Succeeding Growth of Entomogenous Nematode, Steinernema feltiae (str. DD-136), in Spodoptera litura (Lepidoptera : Noctuidae) Larvae