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An artificial diet containing 2% L-ascorbic acid was given to silkworm (Bombyx mori) larvae throughout larval life, or only in some larval instars in order to make a comparison of larvae fed on a diet without L-ascorbic acid throughout larval life. Obtained results show that, when complete L-ascorbic acid deprivation is done during the larval cycle, it affects larval growth and cocoon production. Furthermore, L-ascorbic acid absence from larval food, particularly during the first and last instars, generates beneficial effects to cocoon production without affecting the survival rate or delaying the larval cycle.

applied entomology and zoology

Artificial diet rearing system for the silkworm Bombyx mori (Lepidoptera: Bombycidae): effect of vitamin C deprivation on larval growth and cocoon production