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The investigation was designed to trigger the complete chemical constituents present in the methanolic extract of leaves of hair knot plant; botanically known as Pergularia daemia belongs to the family of the Asclepiadaceae one of the endemic plants of Andhra Pradesh, India by Agilent Technologies Gas Chromatography–Mass Spectrometry and components were well separated on HP-5 MS capillary column consisting of stationary phase of 5% phenyl 95% methylpolysiloxane. The resulted compounds were appropriate to the NIST (National Institute of Standards and Technology) library and the study indicated the presence of different phytochemical compounds. A total of 51 compounds were recognized in the methanolic extract of Pergularia daemia leaves. Further, five new compounds were separated and four of biological importance viz. Pentadecane, Tetratriacontane, Dibutyl phthalate and Squalene were identified. The results of the importance of biologically active phytochemicals in the study recommend, P. daemiaas as a plant of phytopharmaceutical reputation.

Separation and Identification of New Phytocomponents in Methanolic Extract of Leaves of Hairknot Plant (Pergularia daemia) by GC-MS Analysis