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Apigenin and its derivatives are biological active flavonoids that are useful in a variety of dietary constituents. These flavonoids may exert some influence over the transition from normal to cancerous, and have value as chemopreventive substance. In this study, a new purification method of three apigenin glycosides flavonoids from Indian plantation white sugar extracts was developed. Three unknown sugar flavonoids were isolated from sugar by using resin based column chromatography. After filtration, the colorant solution was adsorbed on to the gel column at a flow rate of 1 mL/3 min and elution was done with water at the same rate. 10 mL fractions were collected which were then chromatographed on cellulose TLC plates. The pure fractions were completely evaporated and investigated for identification. The detected flavonoids were: apigenin-8-C-b-Dglucopyranoside, apigenin 6-C-b-glucopyranoside and apigenin-7-O-b-glucopyranoside. Ultraviolet and nuclear magnetic resonance spectroscopy introduces an additional analytical dimension for the identification of sugar flavonoids. keywords: Flavonoid, Sugarcane Plant Extract, Extraction, Resin.

Isolation and Identification of Anticancer Apigenin Glycosides Flavonoids from Plantation White Sugar