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The antibacterial activity of Pseudelephantopus spicatus (Juss) Rohr from the West sub-catchments of Mt. Isarog, Panicuason, Naga City Philippines were investigated. Samples of the plant were collected from wet (PSW-W) and dry (PSW-D) seasons. Ethanolic crude extracts obtained from air-dried leaves of PSW-W and PSW-D samples exhibited antibacterial activity against two Gram-positive bacteria (Micrococcus luteus and Staphylococcus aureus) and two Gram-negative bacteria (Escherichia coli and Enterobacter aerogenes). Assay-guided purification using normal-phase column chromatography and subsequently preparative TLC yielded several active fractions that are bioactive – PSW-D-F-9 and PSW-W-D-13). Phytochemical screening by TLC revealed that the active fraction PSW-W-D-13 contains alkaloids, higher alcohols, phenols, steroids and essential oils while the active fraction PSW-D-F-9 contains tannins, flavonoids, alkaloids, higher alcohols, phenols, steroids and essential oils.

Antibacterial Activity of Pseudoelephantopus spicatus (Juss) Rohr Extracts from the West Sub-catchment of Mt. Isarog Natural Park