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Nanotechnology has emerged as one the most prominent field of study among researchers nowadays and nanoparticles have the potential to be a viable and good option in combating bacterial resistance that has become a growing concern among health practitioners. Silver nanoparticles in particular have been in the spotlight due to its unique properties and excellent antimicrobial properties. Current study reported the synthesis of silver nanoparticles from the aqueous extract of Hibiscus sabdariffa plant belongs to family malvaceae. Addition of silver nitrate to cell free extract showed quickly change in color that indicates the production of nanoparticles. The UV-Visible spectroscopy analysis showed the absorption peak at 422.15 nm that confirms the presence of silver nanoparticles. TEM analysis revealed spherically shaped nanoparticle, well distributed with size ranges from 19 mm to 50 mm. The synthesized silver nanoparticles showed good antibacterial effect when tested against Salmonella enterica, Escherichia coli, Bacillus cereus and Staphylococcus aureus. These nanoparticles showed good synergistic effect along with levofloxacin and amikacin.

Plant Generated Silver Nanoparticles and their Antibacterial Effect in Combination with Levofloxacin and Amikacin