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A novel approach was used to develop and validate a bioanalytical RP-HPLC method for the simultaneous estimation of Paracetamol and Cefixime in rabbit plasma using Cefaclor as internal standard. Evaluation of the content of drugs were done by employing a mixture of Phosphate buffer (p H 6.4) and Acetonitrile (80:20, v/v) as the mobile phase and measure the absorbance at 245 nm for Paracetamol and Cefixime. Retention time was established to be 3.618 min for Cefaclor, 4.608 min for Paracetamol and 5.914min for Cefixime. The results shown that the analytical technique furnished here establishes acceptable accuracy and precision, shorter and easy sample preparation, reduced the complications for equipment on satisfactory analysis time.

Bioanalytical Method Development and Validation for Simultaneous Estimation of Paracetamol and Cefixime by using RP-HPLC in Rabbit Plasma