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A reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Hydrochlorothiazide, Amlodipine Besylate and Telmisartan in pharmaceutical formulation using RP - C18 column. The mobile phase (acetonitrile: acetate buffer adjusted to pH 5 with orthophosphoric acid) was pumped at a flow rate of 1.0 mL min -1 in the ratio of 60:40% v/ v and the eluents were monitored at 333 nm. Linearity was obtained in the concentration range of 20 – 100 µg mL -1 for hydrochlorothiazide, amlodipine besylate and telmisartan. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method can be applied for determining hydrochlorothiazide, amlodipine besylate and telmisartan in bulk and in pharmaceutical dosage form.

oriental journal of chemistry/oriental journal of chemistry

Development and Validation of Rp - Hplc Method for the Simultaneous Determination of Hydrochlorothiazide, Amlodipine Besylate and Telmisartan in Bulk and Pharmaceutical Formulation