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A simple, economical and ecofriendly method for simultaneous quantification of Levofloxacin hemihydrate (LVF) and ambroxol hydrochloride (ABH) has been developed which is found to be accurate and precise. It is based on the first derivative spectrophotometric method and the two wavelengths selected for the quantification of ABH and LVF were 255.70nm (zero crossing point for LVF) and 353nm (zero crossing point for ABH) respectively. 0.1N urea is used as solvent for dissolving the sample as well as for dilution. The first derivative amplitude-concentration plots were linear over the range of 5-40µg/ml and 3-10.5µg/ml with detection limits of 0.25 and 0.3025 µg/ml and quantification limits of 0.833 and 1.008µg/ml for LVF and ABH respectively. The % assay value was within the range of 99.57-102.0%. Since the % relative standard deviation for precision and accuracy of the method was found to be less than 2%, the method can be employed for the quality control tests of studied drugs in their formulations.

oriental journal of chemistry/oriental journal of chemistry

A green analytical method for the simultaneous estimation of Levofloxacin hemihydrate and ambroxol hydrochloride using hydrotropy and first derivative UV spectroscopy