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Nowadays by becoming cognizant of the effects of antimicrobial herbs is increasing according to the use of these plants in the world. Mentioned treatment method cause to reduce the side effects and prevention of bacterial resistance to antibiotics. In this regard, the main focus of the paper is centered on Melissa officinalis aqueous Lemon balm extract antibacterial effect of lamiaceae family on Aeromonas hydrophila factor in vitro condition and it cause hemorrhagic septicemia disease in Oncorhychus mykiss. A. hydrophila of rainbow trout with septicemia symptoms were studied after isolation and culturing in different environments in order to definitive diagnosis by using biochemical and molecular (PCR) tests. In order to determine extract antibacterial power; we have used broth micro-dilution ( liquid dilution), minimum bactericide concentration ( MBC) and minimum inhibition concentration (MIC) standard methods. Then, MIC concentration was used in MULLER Hinton agar by creating wells and it was compared zone diameter of bacterial growth inhibition. Results showed that M.Officinalis aqueous lemon balm extract with MIC amount is equal to 2.5 mg/ml and MBC amount is equal to 5 mg/ml. Results domestrated the positive eefect of the extract on bacteria and in order to use it as a medicine, treatment and prevention of fish disease, accomplishment of further clinical research is required.

biomedical and pharmacology journal/biomedical and pharmacology journal

In vitro Study of the Effect of Melissa Officinalis Aqueous Lemon Balm Extract on Aeromonas Hydrophila Causative Hemorrhagic Septicemia Disease in Oncorhychus Mykiss