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1) To compare qualitative and quantitative profile of herpes viruses, In gingival cervicular fluid of periodontitis and healthy periodontal tissues ; 2) To quantitatively assess the relation between HSV and periodontitis Material and Methods: 40 patients with periodontitis(P), 40 patients with gingivitis (G)and 40 with Healthy gingival tissues (HP) were recruited. GCF samples from periodontal sulcus were analyzed using quantitative real-time PCR to detect and quantify HSV virus. Frequencies of detection and levels of HSV were compared between healthy and diseased. Correlation between HSV viruses and Clinical parameters was assessed. Among the HSV viruses ,HSV 1 prevalence was slightly higher in periodontitis sites compared with controls , EBV-1 and HCMV viruses were less dominant.Significant correlation was seen between the HSV and periodontitis clinical parameters. Prevelance of HSV viruses in GCF and its correlation between the clinical parameters may represent an unspecific indicator for the host response in periodontitis

Estimation of Load of Herpes Viruses In GCF and its Impact on Clinical Parameters in Periodontitis