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Chemiluminescence immunoassay of estrogen was performed. Ovaries were removed and immersed in Bouins solution for fixation and processed until the embedded in paraffin for histological analysis. Data was statistically analyzed by one way analysis of variance (ANOVA) by F –Test. The value p< 0.05 considered as significant. In our study it is observed that, Piper nigrum found much effective to compensate the level of estrogen to normal. This study suggests that the antituberculosis drugs in combination with Piper nigrum are effective to cure the disease and to maintain the fertility in rats. In the present investigation, the increased level of estrogen may be because of zinc and antioxidant property of Piper nigrum, which may also be responsible for the improvement of histological architecture of ovary of Sprague-dawley rats.

Effect of Piper Nigrum (Linn.) on Infertility Induced by Ethionamide and Para Amino Salicylic Acid in Female Sprague –Dawley Rats