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In order to find out another strategy in treating cancer instead of drugs and surgical treatment, therefore, this study was designed to prove that: Firstly: the laser was a safe tool in treating cancer by increasing the number of blood vessels of the spleen without any side effects. Secondly: the laser was effective and successful in treating cancer by increasing blood supply through increasing the number of blood vessels of the spleen in diseased mice with cancer by exposure of the spleen to laser irradiation. Thirty two normal adult mice were selected randomly for this investigation. The mice were divided into four groups (n=8 each).The age of mice was50 days and body weight 3744gm. Group A includes normal control mice non irradiated with laser, group B includes normal mice irradiated with laser, group C includes diseased mice with cancer non irradiated with laser, and group D includes diseased mice with cancer irradiated with laser. Both groups C, and D of mice were inoculated with mammary gland carcinoma, while groups of mice including B, and D were anaesthetized and irradiated with GaAlAs diode laser that directed to the spleen of mice for one hour twice daily at time interval of two hours during the entire period of exposure to laser irradiation (experimentation) which was 4 weeks. At the end of the experiment, all the mice were sacrificed and the spleen was quickly obtained. Sections were prepared by using a routine procedure and examined by light microscopy. The number of blood vessels for overall sections was counted by using mesh lens inserted into eyepiece of the light microscopy. There was a gradual increase in the range of the number of blood vessels of the spleen for normal mice irradiated with laser that reached to (10-13) in the fourth week of the experiment (the last week). There was also a gradual increase but fewer in the range of the number of blood vessels of the spleen for diseased mice with cancer irradiated with laser in comparison with the range of the number of blood vessels of the spleen for normal mice irradiated with laser, the range reached to (10-12) in the fourth week of the experiment. While, in the group of diseased mice with cancer non irradiated with laser, there was a decrease in the range of the number of blood vessels of the spleen in the fourth week of the experiment that was (7-7) when compared to the group of normal control mice non irradiated with laser which the range was (8-8) in the fourth week of the experiment (the last week). Spleen of both diseased mice with cancer and normal mice responded to the laser irradiation and brought about an increase in the number of blood vessels and as a result of this, there was an increase in the blood supply and that led to overcome the cancer, not to be doubted.

Quantitative Study of the Effectiveness of Soft Laser on the Activity of the Blood Vessels in the Spleen of Diseased Mice with Cancer