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Potato (Solanum tuberosum), one of the indispensable food crops, is susceptible to various fungal phyto-pathogenic infections that result in considerable production losses both in terms of quality and quantity. Developing fungal-resistant cultivars by introducing pathogenresistant genes through transgenic approach has been a powerful tool to provide defense against the fungal pathogens. The current study was undertaken to develop a fungal resistant trait in a local potato variety.To achieve this goal, a local Pakistani Potato variety (Diamant), was transformed with chitinaseChiCgene utilizing plasmid pEKB/ChiC using Agrobacterium tumefaciens strain EHA101. The infected explants were grown on MS medium supplemented with 2 mg/l BAP and 0.2 mg/l NAA. Explants were then sub-cultured on MS medium supplemented with 2mg/l BAP and 2mg/l GA3 for shooting. PCR examination confirmedthe integration of ChiC gene in the potato genomic DNA. The transformed potato variety could also be used as fungusresistant breeding material and offers new opportunities to develop improved potato cultivars fordifferent agronomic and other desirable traits. .

ChiC Gene Enhances Fungal Resistance in Indigenous Potato Variety (Diamant) Via Agrobacterium-Mediated Transformation