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In case of Sitopbilus oryzae, Periplaneta americana and Gumbusia affinis (girard), 100 % mortality was noted after 24 hours of exposure to the crude extracts and the isolated compounds where as in control group 100% survival was noted. In the initial stage of the experiment on Sitopbilus oryzae, after 30 minutes of exposure, schooling behavior was observed. After 30 minutes the weevils were dispersed there after towards the corners of the petridishes. After 1hour the locomotary movement of the weevils was restricted and these weevils could not be moved afterwards. The Periplaneta americana showed cleaning behavior at the initial stages of poisoning by rubbing their bodies with their legs or sides of the cages. At the later stages of poisoning Periplaneta americana shows incoordination and convulsion leading to paralysis. Gumbusia affinis showed hypersensitivity, an increased respiratory rate by indicating rapid operculum movement. The fishes were become restless and the movement of the body and operculum of fishes becomes lower down and the fishes were settled down at the bottom of the aquarium. At the final stages of intoxication after 24 hours the fishes were dead and found floating on the surface of the aquarium. The area of the brain of dead fishes was found red in color. From the above symptoms it was evident that the toxin found in the crud extract and the isolated compounds of Tetraodon fluviatilis may contains strong toxin.

Studies on the Pesticidal Activities of Bioactive Compounds of Fish Tetraodon fluviatilis of West Coast of Mumbai