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The present study was undertaken to assess the effectiveness of isolates of Trichoderma asperellum isolates against the chickpea wilt pathogen Fusarium oxysporum f. sp. ciceri. The in vitro assay revealed that there was increase in growth of test pathogen at different intervals of time but marked suppression of mycelial growth was recorded in 96 hrs under dual culture by Trichoderma asperellum isolates. There was significant growth suppression of test pathogen in culture filtrates of isolate-4 throughout the studied time compared to rest of the isolates in poison-food method. Less chlorophyll content was observed in all the culture filtrate treated plants as compared to control, in Fusarium oxysporum f. sp. ciceri (FOC) un-inoculated plants. However, after inoculation of FOC in soil, there was marked increase in chlorophyll content in culture filtrate treated plants as compared to control, with exception to isolate-8 and -10 treated plants. Minimum wilt incidence was recorded in three isolates such as isolate-1, -2 and -4 treated plants while the maximum was recorded in control. Therefore, from the present study it is concluded that the different isolates of T. asperellum showed their different abilities to check the growth of pathogenic fungi under in vitro and in vivo conditions.

Evaluation of Rhizosphere Isolates for Management of Fusarium oxysporium f. sp. ciceri of Chickpea (Cicer arietinum L.)