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-1 AgNO3, and further increase in the concentration of AgNO 3 decreased the shoot production and shoot length. Additionally, we evaluated the effect of various concentrations (0, 10. 30, 50, 100, 200 mg L -1 ) of putrescine on shoot regeneration and shoot length. The highest number of shoots (7.4 ± 0.8) and longest shoots (2.2 ± 0.1 cm) were observed in cultures supplemented with 50 mg L -1 putrescine and further increase in concentration reduced the shoot regeneration and elongation. Our present study suggested that the addition of ethylene inhibitor AgNO 3 and polyamine putrescine could be applied in micropropagation and plant transformation protocols of P. tinctorium.

Silver Nitrate and Putrescine Enhance in vitro Shoot Organogenesis in Polygonum tinctorium