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The objective of the study was to investigate the differences in protease productions of mutans Bacillus licheniformis F11. In this study B. licheniformis F11.1 (DchiA; Dpga) and B. licheniformis F11.4 (DchiAB; Dpga) was used. Two types (LB and MLB + collagen) of media were used for bacterial growth, protease production and zymogram analysis : Luria Broth (LB) and Modified Luria Broth plus collagen (MLB + collagen) media. The LB media contained tryptone 1%, NaCl 1% dan yeast extract 0.5%. The Modified LB + collagen media contained tryptone 0.5%; NaCl 1%, yeast extract 0.25% and collagen 5%. Two protease producing B. licheniformis F11 mutans were screened from Palembang Indonesia, Bacillus licheniformis F11.1 (DchiA; Dpga) and Bacillus licheniformis F11.4 (Dchi AB; Dpga). Both mutants showed different growth pattern and protease production in media containing collagen. F11.1 displays a higher protease activity than F11.4 in Luria Bertani media with an optimum after 15 hours for F11.1 and 15-25 hours for F11.4. Upon addition of collagen to LB medium F11.4 overmatched F11.1 with respect to collagenolytic activity. The presence of collagen led to a shift in the substrate preference of the two strains towards collagen compared to other protein substrates (gelatin, fibrin and casein). Zymogram analysis on gel embedded substrates suggests a higher molecular variation for strain F11.4.The different genetic mutation of the same species (Bacillus licheniformis F11.1 and F11.4) displayed different protease production patterns and substrate specificities especially on collagen.

Differences in Protease Expression of Mutants Bacillus licheniformis F11