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Separation technique of the circulating tumor cell (CTC), which would be a sample for diagnostics of cancer, is important. In the present study, a micro flow device, which has gaps between micro-cylinders, has been fabricated by photolithography technique to distinguish between cancer cells and red blood cells. Two kinds of cells were used in the test: Hepa1-6 and swine red blood cell. The mean diameters of two kinds of cells measured by ImageJ were 21 μm and 6 μm. Variation was made on the dimension of the gap: 10, 15, 20 and 25 μm. The micro cylindrical pattern was fabricated on the slide glass with a photomask of titanium film. The negative resist material (SU-8) was coated on the glass plate to make the 60 μm height of the micro cylindrical pattern. A micro flow channel was made between the glass plate with the micro cylindrical pattern and the flat polydimethylsiloxane (PDMS) disk. Swine blood was diluted 100 times by the saline solution. The concentration of Hepa1-6 was adjusted to 5000 cells/ml by the physiological saline solution or by the swine blood. The cell suspension of 30 μl was injected into the channel by a micropipette, and the trap rate at the gap of the micro pattern was measured. The experimental result shows that trap rates of micro cylindrical pattern gap of 10, 15, 20 and 25 μm are 100, 80, 37 and 26%, respectively. Most of the swine red blood cells can pass through the 10 μm gap. The result shows that the microchannel device developed in the present study is effective for the separation of the CTC.

Trapping of cancer cell by gap of micro cylindrical pattern