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High density culture is one of the essential technologies for an engineered tissue where cellular physiological functions such as enzymatic reactions are required. Cellular energy metabolism and adherence to scaffold are the most important factor to design high density culture. In this study, we developed flow chamber with which steady state measurement of glucose and oxygen metabolic rate of HepG2 cell line can be measured. In addition, cell adhesion ratio was measured using the same flow chamber. The results provided not only glucose and oxygen consumption rates to glucose concentration, but also the interaction of both consumption rates on glucose concentration. Cell adhesion ratio under various shear stresses showed that HepG2 cells are detached under the shear stress around 10-60 Pa. This large diversity of detached shear stress suggests that the adhesion force of collagen fibers to substrate may dominate the detachment.

Steady Measurement of Glucose and Oxygen Consumption Rate and Cell Adhesion Ratio of HepG2 Hepatoma Cells for High Density Culture