English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

(GUV細胞の電気融合による凍結 ・乾燥保護物質の高効率導入に関する研究) 白 樫 了*1, Randolph REUSS*2, Alexander SCHULZ*2 VladimirL. SUKHORUKOV*2, Ulrich ZIMMERMANN*2 Evaluation of FEM Electric Field Analysis Using Electric Properties of Giant Unilamellar Vesicle (GUV) /Jurkat Cell Measured by Electrorotation (Spectroscopy : Introduction of Cryo-and Lyoprotectants by Electrofusion of GUV and Living Cell) Ryo SHIRAKASHI*3, Randolph REUSS, Alexander SCHULZ, Vladimir L. SUKHORUKOV and Ulrich ZIMMERMANN *3 Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505 Japan Electrofusion of Giant Unilamellar Vesicle (GUV) and living cell is a gentle method to deliver membrane impermeable cryo-/lyo-protective molecules into living cell for cryo-/lyopreservation. In this method, optimal length and strength of electric pulse are required to initiate GUV-cell fusion. Calculating electric field by finite element method (FEM) might be powerful analytical method to predict optimal pulse length and strength of a deformed and adhered GUV-cell. The objective of this study is to clarify whether FEM model of pulsed GUV or cell could be applicable to predict its electric field, especially the change of membrane potential. First, electric properties of GUV and Jurkat were derived from electrorotation spectra with dipole approximation model. Secondly, the electric field and the membrane potential of single GUV or cell after applying a stepwise electric field were calculated by FEM model using the measured electric properties. At the first stage after applying a step electric field, the calculated membrance potential by FEM model shows good agreement with the membrane potential derived from the dipole approximation model. At the time after complete relaxation of electric field, calculated membrane potential of FEM model is lower than that of dipole approximation model. This might be because both models do not take effects of electric double layers on both sides of membrance into account.

nihon kikai gakkai ronbunshū. b

Evaluation of FEM Electric Field Analysis Using Electric Properties of Giant Unilamellar Vesicle (GUV)/Jurkat Cell Measured by Electrorotation (Spectroscopy: Introduction of Cryo- and Lyoprotectants by Electrofusion of GUV and Living Cell)