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In this study, we investigated the protective effect of hydroglycolic crude extract of Thai red Hom–Kularb–Drice ( HKD) rice bran on UVBinduced photoaging of human skin fibroblast. The HKD rice bran extract showed scavenging capacity with an EC50 of 4.37 0.34 mg/mL testing using a DPPH radical scavenging assay. The structural identification of proanthocyanidins compounds was performed by Liquid chromatographyelectrospray ionizationquadrupoletimeof flight mass spectrometry ( LCESI-Q-TOF-MS) in the hydroglycolic HKD extract. These compounds were cyanidin B2 type and ( epi) catechin. The extract showed a no cytotoxic response was showed in human skin fibroblast. Furthermore, the HKD extract ( 20 μg/ mL) exhibited a protective effect in UVB irradiated primary skin fibroblast, reducing MMP-1 ( matrix metalloproteinases1) expression and increasing production of type I procollagen. This data suggests that the CMU J. Nat. Sci. (2020) Vol. 19 (1) 35 HKD rice bran extract has potential for use as a skin antiaging agent in cosmetic products.

Hom–Kularb–Dang Rice Bran Extract for the Prevention of UVB-Damage Against Human Skin Fibroblast