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Serum and Urine Samples Serum samples were obtained from normal subjects, patients with growth hormone deficiency (GHD), acromegaly, and chronic renal failure (CRF). To investigate the effects of IGF-I on serum IGFBP-6, we measured serum IGFBP-6 in normal subjects who received sc administration of IGF-I (0.1 mg/kg) once a day for 7 consecutive days (2). Spot urine samples were collected from two normal adults; 20 mL urine samples were dialyzed against distilled water overnight and lyophilized. The samples were reconstituted with distilled water and then passed through a desalting column (AmpureTM SA). The desalted samples were used for IGFBP-6 Western immunoblot.

Serum Insulin-like Growth Factor Binding Protein-6 (IGFBP-6) Levels in Various Clinical Conditions