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In Brazil, peach production is concentrated in the state of Rio Grande do Sul. Peach seedling production in southern Brazil takes place in the field, at the risk of serious phytosanitary problems. In order to avoid such problems, one alternative is the production of seedlings in environment shelter (greenhouses or nurseries). In such cases, it is necessary to disinfect the substrate to eliminate plant pathogens; however, the procedure eliminates almost all microorganisms, including beneficial ones, such as arbuscular mycorrhizal fungi (AMF) (Fachinello et al., 2004).

The Use of Native Arbuscular Mycorrhizal Fungi from Orchards for the Production of Seedlings