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Figure 1. RT-PCR for BCR-ABL fusion transcripts. (A) First (left) and nested (right) PCR for the M-bcr-type transcripts. Lane M, 100-bp ladder for molecular size marker; lane 1, b3a2-type M-bcr case; lane 2, b2a2-type M-bcr case; and lane 3, this case. (B) RT-PCR for the -bcr-type transcripts. Left, RT-PCR using primers for BCR exon 16 and ABL exon 2 (e16a2); and right, RT-PCR using primers for BCR exon 19 and ABL exon 2 (e19a2). Lane M, 100-bp ladder for size marker; lane 2, b3a2-type M-bcr case; and lane 3, this case. The expected sizes of each PCR product are 504 bp and 137 bp. (C) Nucleotide sequence of the PCR products corresponding to the e19a2 BCR-ABL mRNA junction (anti-sense strand sequence). The sequences of PCR primers are presented in the Supplementary Materials (Table S1).

Chronic myeloid leukemia carrying “micro”-bcr breakpoint