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There occurred an incident of respiratory disease at a cattle raising station in Yamaguchi Prefecture during the period from September to October , of 1988. Seventy-seven dairy calves, 60 to 150 days of age, developed respiratory symptoms acconpanied by fever, coughing and nasal discharge. Four cytopathogenic virus strains were isolated in Vero cell cultures from nasal secretions of 12 affected calves and identified as bovine respiratory syncitial virus (BRSV) based on physiochemical properties and results obtained by the fluorescent antibody technique and neutralization tests using a specific immune serum against BRSV. In six of 12 paired serum samples, the neutralizing antibody titer against BRSV increased significantly . The respiratory disease had proceeded unchecked for about a month following the first outbreak . The average duration of the symptoms was approximately 10 days.

Collective Outbreak of Bovine Respiratory Syncytial Virus Infection in Dairy Calves