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For the agar gel immunodiffusion test, bovine leukemia virus antigen was prepared from persistently infected cell culture fluid. It contained envelope glycoprotein (gp) antigen and core protein (p) antigen, the latter being twice as much as the former. An antibody survey was conducted on 453 cattle in the field. In the test, the central well was filled with antigen, two surrounding wells were with positive control serum, and the other four with serum to be tested. As a result, when gp antigen was used after adjusting its titer to 4 units, the best results were obtained and both gp and p antibody could be detected. Both antibodies were very low in titer in the positive serum. Cattle positive for p antibody were only 19.7 % of those positive for gp antibody.

Optimal Titer of Bovine Leukemia Virus Antigen for Immunodiffusion Test