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Bromodeoxyuridine (BrdU) labeling of S-phase cells, which are DNA- synthesizing cells, was demonstrated in 30 canine and 6 feline neoplasms. Simultaneously, the optimization of the BrdU-labeling method, including tissue fixation, administration dose of BrdU, and the immunohistochemical procedure, was examined. BrdU-incorporated cells in a neoplasm of a dog administered 3 mg/kg of BrdU could be detected. Although BrdU-positive cells could be visualized in 70% ethanol fixed samples, we could satisfactorily demonstrate BrdU-immunoreactivities in samples fixed in 10% buffered formalin and subjected to enzymatic treatment and acid hydrolysis. The BrdU-labeling index (LI) of malignant neoplasms tended to be higher than that of benign neoplasms. The mean BrdU LI of malignant neoplasms was significantly higher than that of benign neoplasms (P<0.05). The present study demonstrated that the BrdU-labeling method may be useful for detecting S-phase cells in canine and feline tissues.

Immunohistochemical Demonstration of S-phase Cells in Canine and Feline Spontaneous Tumors by Anti-bromodeoxyuridine Monoclonal Antibody