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Wistar-Furth-Osaka (WF/Osp) rat strain has been considered to induce spontaneous gastro-colonic adenocarcinoma. ACI rats, fosterbred by WF/Osp had developed the same type of colon adenocarcinoma. The serum from colon cancer-carrying WF/Osp rats induced the same tumors in Wistar/Shionogi (Wistar/Shi) rats. The serum from colon cancer-carrying WF/Osp rats also induced the analogous tumors in ACI rats by intra-peritoneal injection at newborn period respectively. Negative staining on solid-phase immune electron microscopy study revealed numerous particles in the tissue culture medium of transplantable gastric carcinoma. To bring to light, the amino acid sequence of virus antigen protein, using affinity chromatography column, we succeeded to obtain pure antigen on PVDF membrane. Protein structure of the antigen was analyzed, and amino acid sequence of Valine-Proline-Asparatic acid-Lysine-Threonine-Valine-Lysine-Tryptophan-Asparagine (VPDKTVKWN) turned out to be the novel structure among protein sequence by FASTA E-mail Server on GenomeNet.

A Part of Amino Acid Sequence of the Gastro-colonic Cancer Inducing Virus Protein in WF-Osaka (WF/Osp) Rats.