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A total of 18 sterile heifers, their mother cows, their half-sib cows and a bull which may or may not be a full-sib were cytogenetically investigated. Three cases out of the 18 sterile heifers showed different types of chromosomal abnormalities respectively; 60, XY; 60, XX/60, XY; 61, XX, +20. The remainder showed normal karyotype.

Cases of XY female, single-birth freemartin and trisomy (61, XX, +20) observed in cytogenetical studies on 18 sterile heifers.