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A retrospective study for the detection of porcine circovirus 2 (PCV-2) DNA was conducted by nested PCR method in 16 cases of swine post-weaning multisystemic wasting syndrome (PMWS) in Thailand. Histopathology showed characteristic lesions of PMWS and intracytoplasmic viral inclusion bodies in macrophages infiltrating in lymphoid tissues. PCV-2 DNA was detected from formalin-fixed and/or formalin-fixed paraffin-embedded tissues from all pigs with PMWS. The amplified products were digested with Hae III.

journal of veterinary medical science

Detection of Porcine Circovirus 2 (PCV-2) DNA by Nested PCR from Formalin-Fixed Tissues of Post-Weaning Multisystemic Wasting Syndrome (PMWS) Pigs in Thailand.