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Using the triple-stain technique, we investigated whether sperm acrosomes in frozen canine semen were protected during freezing and thawing by addition of a surfactant, Orvus ES Paste (OEP), to the extender. Acrosomes were clearly shown to be protected by the addition of OEP to the entender when compared with those in sperm frozen without OEP addition (p&lt;0.05).

Effect of Addition of Orvus ES Paste to Frozen Canine Semen Extender on Sperm Acrosomes.