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Japanese Black fattening cattle in Miyazaki prefecture, Japan were examined for serum vitamin A (V. A) and vitamin E (V. E) by automatic column-switching high performance liquid chromatography with automated deproteinization. Results indicated that most Japanese Black fattening cattle in Miyazaki prefecture may be provided with V. A supplement and diets including little V. E, moderate beta-carotene and V. A during the early fattening stage, and diets including little beta-carotene, V. A and V. E during the middle and later fattening stages. Therefore, monitoring serum V. A and V. E in Japanese Black fattening cattle throughout the fattening period seems necessary for farmers in Miyazaki prefecture to avoid economic loss attributable to these deficiencies.

journal of veterinary medical science

Serum Vitamin A and Vitamin E in Japanese Black Fattening Cattle in Miyazaki Prefecture as Determined by Automatic Column-Switching High Performance Liquid Chromatography.