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Experimentally-induced perforating wounds in 4 Holstein Friesian lactating cows were sutured using Gambee, continuous 2-layer (Cushing for submucosal layer and continuous horizontal mattress for skin and intermediate layer), separated 2-layer (simple continuous for mucosal layer and vertical mattress for skin and intermediate layer) and 3-layer (simple continuous for mucosal and intermediate layers, simple interrupted for skin) suture patterns. Wound healing was evaluated by radiograph, blood flowmetry, tensiometry, measurement of hydroxyproline content and histopathology. The present results suggest that 3-layer pattern provided the best healing of the entire teat. Mucosal hyperplasia was observed in Gambee and continuous 2-layer pattern while eversion of the skin, presence of suture tracts and greater amount of granulation tissue were observed in continuous and separated 2-layer patterns.

Effect of Different Suture Patterns on Wound Healing of the Teat in Dairy Cattle.