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Cumulus-intact and cumulus-free mouse oocytes were exposed to 7% ethanol for 1, 4 and 7 min, and treated with cytochalasin-B. The activation rate and the proportion of diploid parthenogenones in all groups were not significantly different. After 96 hr in culture, a higher number of blastocysts was obtained when either cumulus-intact or cumulus-free oocytes were exposed for shorter times (1 and 4 min) to ethanol. The presence or absence of cumulus cells at activation had no effect on the percentage of blastocysts. However, at 1 and 4 min ethanol-exposure periods, the parthenogenones derived from cumulus-intact oocytes had a higher number of cells than ones derived from cumulus-free oocytes.

Effect of Cumulus Cells and Exposure Period to Ethanol on In Vitro Development of Mouse Diploid Parthenogenones