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We determined a comprehensive immunohistochemistry of putative isoforms of enzymes for prostaglandin (PG) F 2 α and PGE 2 biosynthesis and these PGs levels in placenta and fetal membrane of normal pregnant rats in vivo. Placenta and fetal membrane showed positive immunoreactions for phospholipase A 2 group 4A, but not group 2A, and cyclooxygenase (COX)-1 rather than COX-2. They showed positive immunoreactions for at least one isoform of each of PGF synthase and PGE synthase with tissue-dependent variations. PGF 2 α and PGE 2 levels in both tissues were highest on day 12 and declined and remained low thereafter. Obtained data would be the basic information on the primary PGs synthesis in rat placenta and fetal membrane in normal pregnancy.

journal of veterinary medical science

Prostaglandins F&lt;sub&gt;2&lt;/sub&gt;α and E&lt;sub&gt;2&lt;/sub&gt; in rat placenta and fetal membrane: a comprehensive immunohistochemistry of their synthetic enzymes and &lt;i&gt;in vivo&lt;/i&gt; tissue levels during normal pregnancy