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To differentiate the avirulent from virulent strains of avian paramyxovirus serotype-1 (APMV-1, Newcastle disease virus), PCR and restriction endonuclease analysis (REA) was employed. Primer sequences were used to amplify a 766-base pair fragment that included the fusion protein cleavage site. REA of PCR products generated by Hin1 I and Apa I enabled the differentiation of avirulent field and vaccine strains from more virulent field strains of APMV-1 in Japan.

journal of veterinary medical science

Simple Differentiation of Avirulent and Virulent Strains of Avian Paramyxovirus Serotype-1 (Newcastle Disease Virus) by PCR and Restriction Endonuclease Analysis in Japan