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The activated sludge process of the digestate liquors after chemical separation wasconducted using a 10 L lab-scale sequencing batch reactor (SBR) and a 0.50 m3pilotscaleSBR independently (with pH control). Due to the relatively high concentrationof free ammonia (FA), clear inhibitory effects of the digestate liquors on the nitrifyingbacteria were observed. The adaptation of the activated sludge to the toxicity wasevaluated with the trends of ammonia uptake rate (AUR) and nitrate utilization rate(NUR). The lab-scale AUR values decreased from 5.3 to 2.6 g N/(kg VSS·h) overtime after the addition of digestate liquors (5–10% of the reactor working volume), indicatingan apparent FA inhibition on the nitrification process in the FA concentrationrange of 0.3–0.5 mg N/L. The pilot-scale AUR values increased from 1.8 to 3.6 g N/(kg VSS·h) in the first two weeks and then decreased to 2.4 g N/(kg VSS·h), showinga lag of the inhibition on the nitrifying bacteria at the FA concentration ≈ 0.15 mg N/L.The lab-scale NURs increased from 2.6 to 10.4 g N/(kg VSS·h) over time, and thepilot-scale NURs increased from 1.0 to 4.0 g N/(kg VSS·h) in a similar pattern. Theclear dependence of both the lab- and pilot-scale NURs on time indicated the adaptationof the heterotrophic biomass to the digestate liquors. Ethanol – used instead offusel oil – was found to be a more efficient external carbon source for better adaptationof the activated sludge under unfavorable conditions.

ADAPTATION OF THE ACTIVATED SLUDGE TO THE DIGESTATE LIQUORS DURING THE NITRIFICATION AND DENITRIFICATION PROCESSES