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The present study dealt with evaluating the effect of different crude extracts from the larvae of palm weevil, Rhynchophorus ferrugineus and cotton leaf worm, Spodoptera littoralis on different pathogenic strains of bacteria and viruses. The results revealed that, ethyl acetate extracts of R. ferrugineus and S. littoralis larvae were more effective against different pathogenic bacterial strains tested than petroleum ether, chloroform, methanol and ethanol extract. Also,The MICs’ determination showed that all different extracts of R. ferrugineus and S. littoralis larvae were active against tested bacterial strains. Minimum Inhibitory Concentration (MIC) values were ranged from 25 to 6.25mg/ml depended on tested species. Ethyl acetate extract from R. ferrugineus and S. littoralis larvae exhibited antiviral activity against hepatitis A virus (HAV) and Herpes simplex virus (HSV-1) cell lines at 625 & 312.5mg/ml. Ethyl acetate extract from R. ferrugineus and S. littoralis larvae recorded 5.19 & 4.07% antiviral activity against HAV at 625 & 312.5mg/ml, respectively; while, antiviral activity percentage was 12.42 & 26.17 at 625 and 312.5mg/ml, respectively against HSV-1. Tested extracts exhibited no antiviral activity against Coxsackie B virus (Cox B4) cell lines. Crude extracts of R. ferrugineus & S. littoralis were not cytotoxic to Vero (CCL-81) cells at 312.5 and/or 625μg/ml.

journal of the egyptian society of parasitology/journal of the egyptian society of parasitology 

ANTIBACTERIAL, ANTIVIRAL AND CYTOTOXIC ACTIVITIES OF RHYNCHOPHORUS FERRUGINEUS (COLEOPTERA: DRYOPHTHORIDAE) AND SPODOPTERA LITTORALIS (LEPIDOPTERA: NOCTUIDAE) LARVAL EXTRACTS