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1Living diatoms show a promise for applications in various fields in particular for the fabrication of micro/nano devices. This study acquired pure diatom walls (frustules) by several steps including collection, separation, and pure culture of marine diatoms. A two-step acid cleaning and baking method was adopted to purify biosilica structures from the diatoms. Results indicated that the morphology of the diatom frustules began to change at a baking temperature of 700 °C. With rising temperatures, the damage to the diatom frustules was more severe, but the composition was less affected by temperature. Therefore, an optimal parameter of 600 °C, 1 °C min-1, and 2 h was set for our study. However, because of the species-specific differences, the morphology and composition of the different frustules were not identical at the optimal parameter.

Influence of Baking and Acid Cleaning on the Morphology and Composition of Diatom Biosilica