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Rapeseed (Brassica napus ssp. oleifera) is described mostly as a good source of plant material to conduct in vitro cultures, however the species reveal considerable genotypic diversity concerning regeneration ability. The aim of this experiment was to analyze regeneration capacity of eleven closely related rapeseed doubled haploid lines under in vitro conditions. Cotyledons and hypocotyls were incubated on a standard MS medium with the addition of 10mg/l indole-3-butyric acid. MS medium without auxin was used as the control. Regeneration assessment was done after 28 days of culture. Genotypic variety regarding regeneration effectiveness was observed among the tested DH lines. Statistical tests showed significant differences in shoot regeneration in 4 out of 11 DH line pairs incubated on the control medium and in 8 pairs incubated on the medium containing phytohormone. Closely related DH lines revealing significant genotypic shoot regeneration diversity may be useful in further research on the genetic background of rapeseed regeneration ability under in vitro conditions.

Regeneration ability analysis of closely related doubled haploids of rapeseed (Brassica napus ssp. oleifera).