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Present study aimed to determine the possibility of cleared-ovule technique adaptation for early embryo development in Linum (Linum usitatissimum L.) observation. This method was primarily introduced in cereals by Ponitka and Ślusarkiewicz-Jarzina [1]. Flax and linseed seed capsules were sampled after 10 and 15 days since the beginning of flowering. In the ovules isolated, different development stages of embryos were observed. The results obtained suggest, that clearedovule technique may be easily applied, not only for Linum embryo development observation, but also as an alternative method for time-consuming and expensive paraffin-embedded technique.

Cleared-ovule method used for early embryo development in flax observation (Linum usitatissimum L.)