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To overcome infertility of male transgenic mice with low-motility spermatozoa, we examined the benefits of the zona pellucida incision using a piezomicromanipulator (ZIP) on in vitro fertilization (IVF) using low-motility spermatozoa. The incision was about 26 μm long, representing 8% of the perimeter of the zona pellucida of the mature oocyte. Over 95% of cumulusfree oocytes survived after ZIP. The in vitro fertilization rate of ZIP oocytes by low-motility spermatozoa of the transgenic mice was 22–57%, compared with 1–2% of cumulus-intact oocytes. After embryo transfer of ZIP zygotes at 2-cell stage into the oviducts of recipients, 11–20% of the transferred embryos developed to term. Our results indicate that ZIP is a useful technique for IVF of ova by low-motile spermatozoa and subsequent embryo transfer.

journal of mammalian ova research

Partial Incision of the Zona Pellucida using Piezo-micromanipulator Improves In Vitro Fertilization using Low-motility Spermatozoa of Infertile Transgenic Mice