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It is known that many fungal species have chitin as a main component of the cell wall. In higher plants, chitinases have been implicated in the defense reaction against pathogens, because they directly attack the cell wall and inhibit fungal growth in vitro.1>2) However, since the fungal cell wall contains chitosan to some extent,3) it is uncertain whether chitinase indeed functions in the degradation of the cell walls of pathogens. In order to efficiently control the fungal plant-pathogen, therefore, it is important to confirm the structural details of the chitinous component in the intact cell wall by a nondestructive method. Solid state cross-polarization magic-angle spinning (CP/MAS)NMRspectroscopy is one of the most useful methods for structural elucidation under nondestructive conditions, and has been applied to the structural analyses of the bacterial cell wall,4) insect cuticle,5) and other insoluble materials. In this paper, we report the CP/MAS 1 3C-NMRspectrum of the cell wall ofFusarium oxysporum f. sp. lycopersici race 7, and discuss the structure of the cell wall and its decomposability by the chitinases. Dried F. oxysporum cells were suspended in distilled water and completely ground in a mortar with a pestle. After confirming cell destruction under a microscope, the homogenate was centrifuged at 3500# for 20min. The precipitate was washed with distilled water and then lyophilized. Chitin (0% deacetylated) and chitosan 70M (65-75% deacetylated) were purchased from Wako Pure Chemical Industries Ltd., and were used as references. CP/MAS 13C-NMRspectra were obtained at 67.8 MHz by using a JNM-GSX 270 spectrometer with 5ms cross-polarization transfers from protons, a 27kHz

Solid state 13C-NMR analysis of cell wall components of Fusarium oxysporum.