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The yam tuber is a high class vegetable in Japan. We have found chitinase in yam tubers, and electrophoretically purified the enzyme in high yield.0 Yam chitinase is useful for preparation of protoplasts from some basidiomycetes such as Tricholoma matsutake, Lentinus edodes, and Lyophyllum shimeji.2) Recently, the physiological role of plant chitinase has been proposed to be self defense against plant pathogens.3l4) To study the induction of

Callus induction from yam, Dioscorea opposita thunb., and the subculture.