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The phenolic acids found in graminaceous plant cellwalls are esters with sugars and lignin.1 ~3) Previously, we separated two feruloylated oligosaccharides from an enzymatic hydrolysate of the bagasse lignin-carbohydrate complex.4'5* As an extension of the structural elucidation of phenolic-acid-containing oligosaccharides, we now describe the isolation and identification of a new tetrasaccharide mixedly esterified with acetic acid and ferulic acid 1 from sugar cane baggase. Extractive-free and depectinated bagasse from sugar cane (Saccharum officinarum L. NCo310) was treated with a hemicellulase-cellulase mixture as described previously.5) The 80% aqueous ethanol-soluble fraction of the hydrolysate was chromatographed on a Sephadex LH-20 column [eluant, water] to give a mixture of esterified oligosaccharides. A conjugate 1 having an Rfvalue of0.41 was separated from the others by preparative TLC [irrigant, 1-butanol:acetic acid:water=62: 15:23 (v/v)],

Arabinosylxylotriose mixedly esterified with acetic and ferulic acids from suger cane bagasse.