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A simple method has been developed for analysis of gizzerosine in fish meals. A sample, hydrolyzed with 6 n HCl, was transferred into an Amberlite IR CG-50 column which was equilibrated with sodium borate solution (0.045 m, pH 8.25). After elimination of a large portion of amino acids from the column, gizzerosine was eluted with 0.5 n NH4OH and then measured by HPLC. HPLC separation was done with an analytical column of Hitachi 2619-F and gradient elution with three solvents (two borate buffers—pH 9.2 and 10.7, [Na+] = 0.1 m, containing 3% (v/v) EtOH—and 0.8% NaOH). Gizzerosine was measured by the post-column reaction of OPA and fruorometric detection. Recovery of gizzerosine added to fish meal was over 90% and the detection limit was 1 ppm.

Simple measurement of gizzerosine in fish meals by high-performance liquid chromatography.