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Berberine is an important phytochemical known to have antibacterial, stomachic and anti-inflammatory activities. The rhizome of a typical source plant, Coptisjaponica, has a low content ofberberine and more than 5 years growth is necessary before the rhizomes can be harvested. Yamada and Sato succeeded in establishing a methodfor berberine production by cell cultures of Coptisjaponica} >2) Wehave undertaken to develop a method for the industrial production of berberine using their cell line. Since Zenk et al.3) showed that a large amount ofindole alkaloids could be produced in a two-stage culture of

Berberine production by cultured Coptis japonica cells in a one-stage culture using medium with a high copper concentration.