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named Baciitus novosp. strain MN-32, The growth temperature of MN-32 strain is between 50C'C to 70"C (optimum temperature, 60"C), the growth pH range is pH 2.0・-hv7,5 (OPtimum, pH 3.0 A.. 5.0). The proteolytic activity was measured by the casein-Folin method at 70"C, pH 3.0 for 30 min, as reported previously.9) For the production of acid proteinase, the strain was aerobically cultured at 60CC in a medium comprising 1.5% polypeptonc, 0,IVf;1 yeast extract, O,31e/. NaCl, O.5% KH2P04, O.05% MgSO,・7H,O, and 0,O1 9・: CaCr, at pH 3,5. . The procedures for purification of MN-32 acid proteinase were as follows. The culture filtrate (1001) was fractionated with arn-

A novel thermostable, S-PI (pepstatin ac)-insensitive acid proteinase from thermophilic Bacillus novosp. strain MN-32.